ISOZYME PATTERNS ON CELLULOSE ACETATE GEL ELECTROPHORESIS (CAGE) DIFFERENTIATE MEXICAN POTATO CULTIVARS

Abstract

Cellulose acetate gel electrophoresis (CAGE) as a method for cultivar characterization was evaluated for nine enzymes from 16 potato (Solanum tuberosum L.) genetic materials. Two different parts of the plants (tubers and foliage) and growing conditions (in vivo, in vitro) were also considered. For most of the enzymatic systems tested, the bands from tuber were more evident than those from foliage. However, peroxidase showed the highest variation both on the amount and distribution of bands from foliage in vitro than from tuber in vivo. On the other hand, acid phosphatase and alcohol dehydrogenase did not migrate. 6-phosphogluconate dehydrogenase characterized well the cultivar Puebla from the rest of the materials. Glutamate oxaloacetate transferase was useful to detect clone 676082 in samples from foliage in vitro.