POTATO TUBER ISOZYMES IN CELLULOSE ACETATE GEL ELECTROPHORESIS (CAGE)

Authors

  • Héctor Lozoya-Saldaña
  • María Teresa Colinas-León
  • María Sandra Cervantes-Ramírez
  • Jesús García-Martínez

Keywords:

Solanum tuberosum L., tubers, enzymes, cultivar characterization, genetic markers, electrophoresis, CAGE

Abstract

Macerated tuber samples from the potato (Solanum tuberosum L.) cultivars Alpha, Atlantic, Gigant, Ireri, Mundial, and Criolla, were analyzed in cellulose acetate gel electrophoresis (CAGE) for the presence and activity of esterase (EST), peroxidase (PER), glutamate-oxaloacetate-transferase (GOT), malate dehydrogenase (MDH), and 6- phosphogluconate-dehydrogenase (6PGDH). The best differentiating enzyme systems were EST, PER, GOT, and MDH, after 7 and 14 days of storage at 0º C for the cultivars Ireri, Mundial, and Criolla. The presence of contrasting CAGE enzymatic polymorphisms are useful as genetic markers to characterize potato cultivars.

Downloads

Published

30-09-1997

Issue

Vol. 31 No. 3 (1997): 1997-jul-sep

Section

Notes

License

Agrociencia is published every 45 days, in an English format, and it is edited by the Colegio de Postgraduados. Mexico-Texcoco highway Km. 36.5, Montecillo, Texcoco, Estado de México, CP 56264, Telephone (52) 5959284427. www.colpos.mx. Editor-in-Chief: Dr. Fernando Carlos Gómez Merino. Rights Reserved for Exclusive Use: 04-2021-031913431800-203, e-ISSN: 2521-9766, granted by the National Institute for Author Right.